PCR Primer Tm Calculator

Calculate primer melting temperature using multiple methods

Primer Sequence

DNA Sequence (5' → 3')

Calculation Method

Primer Concentration (nM)

Salt Concentration (mM)

Melting Temperature (Tm)

75.9°C

Nearest-Neighbor Method

Primer Properties

Length20 bp

GC Content50.0%

A Count5

T Count5

G Count5

C Count5

All Tm Methods

Basic (Wallace)60.0°C

Salt-Adjusted46.7°C

Nearest-Neighbor75.9°C