PCR Primer Tm Calculator
Calculate primer melting temperature using multiple methods
Primer Sequence
Melting Temperature (Tm)
75.9°C
Nearest-Neighbor Method
Primer Properties
Length20 bp
GC Content50.0%
A Count5
T Count5
G Count5
C Count5
All Tm Methods
Basic (Wallace)60.0°C
Salt-Adjusted46.7°C
Nearest-Neighbor75.9°C
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Editorial Note
MyCalcBuddy Editorial Team
This page is maintained as an educational calculator reference.
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Formula Source: Standard Mathematical References
by Various
🔄Last reviewed: May 2026
✓Formula checks are based on standard references and internal QA review.