EC50 Calculator
Calculate EC50 (half maximal effective concentration) from dose-response data for agonist analysis.
Dose-Response Data
Concentration (uM)
% Response
EC50 Definition
The concentration producing 50% of the maximum effect. Lower EC50 = higher potency.
EC50
1.389 uM
pEC50: -0.14
Pharmacological Parameters
EC501.3895 uM
pEC50 (-log EC50)-0.14
Hill Slope (approx)2.90
Efficacy (Emax)95.0%
Response Parameters
EC20146.78 nM
EC501.389 uM
EC8010.000 uM
Baseline5.0%
Span (Max - Baseline)90.0%
Data Summary
| Conc | % Response |
|---|---|
| 10.00 nM | 5.0% |
| 100.00 nM | 15.0% |
| 1.000 uM | 45.0% |
| 10.000 uM | 80.0% |
| 100.000 uM | 95.0% |
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Editorial Note
MyCalcBuddy Editorial Team
This page is maintained as an educational calculator reference.
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Formula Source: Standard Mathematical References
by Various
🔄Last reviewed: May 2026
✓Formula checks are based on standard references and internal QA review.