Spectrophotometer Calculator
Apply Beer-Lambert Law to calculate concentrations from spectrophotometric measurements.
Measurement Parameters
Beer-Lambert Law
A = epsilon x c x l
A = absorbance, epsilon = extinction coeff, c = concentration, l = path length
Concentration
8.039e-5 M
Results
Concentration (mM)0.0804
Concentration (uM)80.39
Absorbance0.5000
Transmittance31.62%
A = -log10(T/100)0.5000
Common Extinction Coefficients
NADH (340 nm)6,220
DNA (260 nm)50
Protein (280 nm)1
p-Nitrophenol (405 nm)18,500
Bradford dye44,000
Cytochrome c29,500
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Editorial Note
MyCalcBuddy Editorial Team
This page is maintained as an educational calculator reference.
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Formula Source: Standard Mathematical References
by Various
🔄Last reviewed: May 2026
✓Formula checks are based on standard references and internal QA review.