Scatchard Plot Calculator

Analyze receptor-ligand binding data using Scatchard plot analysis

Binding Data Points

Enter your binding data (Bound vs Free ligand concentrations)

PointBoundFree

#1

#2

#3

#4

#5

Single Point Analysis

Bound

Free

B/F = 1.6000

Kd (Dissociation)

4.485e-1

Bmax

1.1641

Ka (Association)

2.230e+0

R-squared

94.11%

Regression Parameters

Slope

-2.2296

Y-intercept

2.5955

X-intercept (Bmax)

1.1641

Scatchard Equation

B/F = -Ka * B + Ka * Bmax

Slope = -Ka = -1/Kd
Y-intercept = Bmax/Kd
X-intercept = Bmax