Saturation Binding Calculator

Calculate specific and non-specific binding from saturation binding experiments

Binding Parameters

Ligand Concentration [L]

Dissociation Constant (Kd)

Maximum Binding (Bmax)

Non-specific Binding Slope

Measured Total Binding (optional)

Fractional Occupancy

66.67%

Specific

66.67

Non-specific

1.00

Total

67.67

% of Bmax

66.67%

Binding Potential

20.00

[L] for Saturation Levels

50%:5.00

80%:20.00

90%:45.00

95%:95.00

Binding Equations

Specific: B = Bmax*[L]/(Kd+[L])
Non-specific: NS = slope*[L]
Total = Specific + NS