Beer-Lambert Law Calculator
Solve for any variable in the Beer-Lambert equation: A = εcl
Solve For
Beer-Lambert Law
A = ε × c × l
A = Absorbance (unitless)
ε = Molar extinction coefficient (M⁻¹cm⁻¹)
c = Concentration (M)
l = Path length (cm)
Concentration
100.0000 µM
All Parameters
Absorbance (A)0.5000
Extinction Coeff. (ε)5,000 M⁻¹cm⁻¹
Concentration100.0000 µM
Path Length (l)1 cm
Transmittance31.62%
Concentration Units
Molar (M)1.0000e-4
Millimolar (mM)0.1000
Micromolar (µM)100.0000
Nanomolar (nM)100000.00
About Beer-Lambert Law
The Beer-Lambert law (also called Beer's law) relates the attenuation of light to the properties of the material through which the light is traveling.
- Linear range: Typically valid for A = 0.1 to 1.0
- Deviations: Can occur at high concentrations due to molecular interactions
- Applications: Quantitative analysis, spectrophotometry, colorimetry