Protein Isoelectric Point Calculator
Calculate pI and net charge from protein sequence
Protein Sequence
Ionizable Residues
Basic (Positive)
Lysine (K)1
Arginine (R)1
Histidine (H)1
Acidic (Negative)
Aspartic (D)2
Glutamic (E)0
Cysteine (C)2
Tyrosine (Y)0
Isoelectric Point (pI)
7.00
Net charge = 0 at this pH
Charge Analysis
Sequence Length41 aa
Total Basic Residues+3
Total Acidic Residues-2
Net Charge (theoretical)+1
Charge at Common pH
pH 7.0+0.00
pH 7.4 (physiological)-0.18
Interpretation
Basic protein - positively charged at physiological pH
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Editorial Note
MyCalcBuddy Editorial Team
This page is maintained as an educational calculator reference.
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Formula Source: Standard Mathematical References
by Various
🔄Last reviewed: May 2026
✓Formula checks are based on standard references and internal QA review.