Spectrophotometer Calculator

Apply Beer-Lambert Law to calculate concentrations from spectrophotometric measurements.

Measurement Parameters

Beer-Lambert Law

A = epsilon x c x l

A = absorbance, epsilon = extinction coeff, c = concentration, l = path length

Concentration

8.039e-5 M

Results

Concentration (mM)0.0804
Concentration (uM)80.39
Absorbance0.5000
Transmittance31.62%
A = -log10(T/100)0.5000

Common Extinction Coefficients

NADH (340 nm)6,220
DNA (260 nm)50
Protein (280 nm)1
p-Nitrophenol (405 nm)18,500
Bradford dye44,000
Cytochrome c29,500