IC50 Calculator
Calculate IC50 (half maximal inhibitory concentration) from dose-response inhibition data.
Dose-Response Data
Concentration (uM)
% Inhibition
IC50 Definition
The concentration at which 50% of the maximum effect (inhibition) is observed. Lower IC50 = higher potency.
Tips
- - Enter at least 3 data points
- - Include points above and below 50% inhibition
- - Use log-spaced concentrations
IC50
13.3 uM
Log IC50: 1.13
Curve Parameters
IC5013.335 uM
Hill Slope (approx)2.38
Concentration Range100.0 nM - 1.0 mM
Inhibition Range5.0% - 95.0%
Interpolation Points
Lower Point10.0 uM = 45%
IC50 (50%)13.3 uM
Upper Point100.0 uM = 85%
Data Summary
| Conc | % Inhibition |
|---|---|
| 100.0 nM | 5.0% |
| 1.0 uM | 15.0% |
| 10.0 uM | 45.0% |
| 100.0 uM | 85.0% |
| 1.0 mM | 95.0% |
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Editorial Note
MyCalcBuddy Editorial Team
This page is maintained as an educational calculator reference.
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Formula Source: Standard Mathematical References
by Various
🔄Last reviewed: May 2026
✓Formula checks are based on standard references and internal QA review.