Apoptosis Calculator

Calculate apoptosis rate using Annexin V/PI flow cytometry, TUNEL assay, or caspase activity.

Apoptosis Assay

Quadrant Percentages

Q4: Live (An-/PI-)

Q3: Early Apoptotic (An+/PI-)

Q2: Late Apoptotic (An+/PI+)

Q1: Necrotic (An-/PI+)

Apoptosis Index

Total Apoptotic = Early + Late Apoptotic

Apoptosis Rate

27.0%

Analysis Results

Live Cells65.0%

Early Apoptotic15.0%

Late Apoptotic12.0%

Necrotic8.0%

Annexin V/PI Quadrants

Q1: An-/PI+

Necrotic

Q2: An+/PI+

Late Apoptotic

Q4: An-/PI-

Live

Q3: An+/PI-

Early Apoptotic