Dose-Response Calculator
Calculate dose-response curves using the four-parameter logistic (4PL) Hill equation.
Curve Parameters
4-Parameter Logistic Equation
Y = Bottom + (Top - Bottom) / (1 + (EC50/X)^Hill)
Predicted Response
50.00%
Normalized: 50.0%
Curve Parameters
EC5010.000 uM
Hill Slope1
Bottom0%
Top100%
Span (Top - Bottom)100%
Dose-Response Reference
| Concentration | Response |
|---|---|
| 10.00 nM | 0.1% |
| 158.49 nM | 1.6% |
| 2.512 uM | 20.1% |
| 39.811 uM | 79.9% |
| 630.957 uM | 98.4% |
| 10.00 mM | 99.9% |
Hill Slope Interpretation
n = 1: Standard hyperbolic response
n greater than 1: Positive cooperativity (steep)
n less than 1: Negative cooperativity (shallow)