Plating Efficiency Calculator
Calculate plating efficiency and cell survival fraction for clonogenic assays.
Plating Data
Basic PE Calculation
Survival Fraction (Optional)
Plating Efficiency Formula
PE = (Colonies / Cells plated) × 100%
SF = PE(treatment) / PE(control)
Plating Efficiency
45.0%
Quality: Acceptable
Calculation Details
Colonies Formed45
Cells Plated100
Colonies Lost55 (55.0%)
PE Quality Guidelines
> 70%Excellent
50-70%Good
30-50%Acceptable
10-30%Low
< 10%Poor
Tips for Better PE
- - Use cells in log phase (60-80% confluent)
- - Gentle trypsinization and handling
- - Accurate cell counting
- - Optimal seeding density
- - Fresh, properly conditioned media