Hill Coefficient Calculator
Determine the Hill coefficient from binding data to analyze cooperative interactions
Two-Point Analysis
Enter two data points from your binding curve (% bound vs concentration)
Hill Coefficient (n)
0.954
EC50
10.0000
Apparent Sites
1
Interpretation
No significant cooperativity (independent binding)
Hill Coefficient Values
- n = 1: No cooperativity
- n > 1: Positive cooperativity
- n < 1: Negative cooperativity
- n = number of binding sites (ideally)