Protein Concentration Calculator
Calculate protein concentration from UV absorbance measurements
Measurement Parameters
Measured Concentration
11.39 µM
0.285 mg/mL
All Units
Molar (M)1.139e-5
Micromolar (µM)11.392
Nanomolar (nM)11392.1
mg/mL0.2848
Beer-Lambert Law
c = A / (ε × l) = 0.5 / (43890 × 1)
About Protein Concentration Measurement
UV absorbance at 280nm is commonly used to determine protein concentration. The method relies on:
- Aromatic residues: Trp, Tyr, and Phe absorb UV light
- Extinction coefficient: Sequence-specific absorption property
- Beer-Lambert law: A = εcl relates absorbance to concentration
For accurate results, ensure absorbance is between 0.1 and 1.0 (linear range).