DNA Concentration Calculator

Calculate nucleic acid concentration and assess purity from UV absorbance

Spectrophotometer Readings

Nucleic Acid Type

A260 (Absorbance at 260nm)

A280 (Absorbance at 280nm)

A230 (Absorbance at 230nm)

Dilution Factor

Path Length (cm)

Concentration

25.00 ng/µL

Using coefficient: 50 (ng·cm/µL)

Purity Ratios

A260/A2802.00

RNA contamination

A260/A2302.50

Check sample

Unit Conversions

ng/µL25.00

µg/mL25.00

mg/mL0.0250

Total (100 µL)2500 ng