DNA Concentration Calculator

Calculate nucleic acid concentration and assess purity from UV absorbance

Nucleic Acid Type

A260 (Absorbance at 260nm)

A280 (Absorbance at 280nm)

A230 (Absorbance at 230nm)

Dilution Factor

Path Length (cm)

25.00 ng/µL

Using coefficient: 50 (ng·cm/µL)

A260/A2802.00

RNA contamination

A260/A2302.50

Check sample

ng/µL25.00

µg/mL25.00

mg/mL0.0250

Total (100 µL)2500 ng

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MyCalcBuddy Editorial Team

This page is maintained as an educational calculator reference.

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Formula Source: Standard Mathematical References

by Various

ðŸ”„Last reviewed: May 2026

âœ“Formula checks are based on standard references and internal QA review.