DNA GC Content Calculator
Analyze nucleotide composition and calculate GC/AT content of DNA or RNA sequences
Enter Sequence
Non-nucleotide characters will be ignored
Nucleotide Counts
Adenine (A)6
Thymine (T)6
Guanine (G)7
Cytosine (C)6
GC Content
52.0%
13 bases
AT Content
48.0%
12 bases
Sequence Statistics
GC Content52.0%
Total Length
25 bp
Type
DNA
GC Skew
0.077
AT Skew
0.000
GC Content Interpretation
Optimal GC content range for most applications
About GC Content
GC content is the percentage of guanine (G) and cytosine (C) bases in a DNA or RNA sequence. It is an important parameter in molecular biology because:
- Thermal Stability: Higher GC content increases DNA melting temperature due to three hydrogen bonds between G-C pairs
- Primer Design: Optimal primers typically have 40-60% GC content
- Genome Analysis: GC content varies between organisms and can indicate evolutionary relationships
- PCR Optimization: High GC regions may require special conditions or additives