DNA Melting Temperature Calculator
Calculate the melting temperature (Tm) of DNA sequences using multiple methods
Input Parameters
Melting Temperature
43.6°C
Sequence Analysis
Length
17 bp
GC Content
52.9%
A / T
4 / 4
G / C
5 / 4
About DNA Melting Temperature
The melting temperature (Tm) is the temperature at which 50% of DNA molecules are in double-stranded form and 50% are single-stranded.
Calculation Methods:
- Basic (Wallace Rule): Tm = 2(A+T) + 4(G+C). Simple but only accurate for short oligos.
- Salt-Adjusted: Accounts for ionic strength effects on DNA stability.
- Nearest-Neighbor: Most accurate method, considers stacking interactions between adjacent bases.
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Editorial Note
MyCalcBuddy Editorial Team
This page is maintained as an educational calculator reference.
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Formula Source: Standard Mathematical References
by Various
🔄Last reviewed: May 2026
✓Formula checks are based on standard references and internal QA review.