RNA Concentration Calculator

Calculate RNA concentration and assess purity from UV absorbance readings

Absorbance Readings

A260 (260nm)

A280 (280nm)

A230 (230nm)

Dilution Factor

Path Length (cm)

Sample Volume (µL)

RNA Concentration

16.00 ng/µL

Total yield: 0.80 µg

Purity Assessment

A260/A2802.00

Pure RNA

Pure RNA: 1.9-2.1

A260/A2302.22

Unusual ratio - check sample

Pure sample: 2.0-2.2

Application Suitability

RT-qPCRSuitable

RNA-seqCheck purity/concentration

Northern BlotMay need concentration