dN/dS Calculator

Calculate synonymous and nonsynonymous substitution rates to detect selection.

Substitution Data

Synonymous (Silent)

Sites (Ls)

Differences (Ds)

Nonsynonymous (Replacement)

Sites (Ln)

Differences (Dn)

Divergence Time (MY)

Interpretation

omega < 1: Purifying selection
omega = 1: Neutral evolution
omega > 1: Positive selection

dN/dS Ratio (omega)

0.6589

omega = dN / dS

Weak purifying selection

Selection Analysis

↓

Purifying Selection

Substitution Rates

dS (synonymous)0.051745

dN (nonsynonymous)0.034097

Raw Data

pS (proportion)0.050000

pN (proportion)0.033333

Syn. Rate (×10⁻⁹/yr)2587232.6808

Nonsyn. Rate (×10⁻⁹/yr)1704839.0279