Transfection Calculator
Calculate DNA and reagent amounts for cell transfection experiments.
Transfection Parameters
Per Well Requirements
DNA
2.5 ug
2.5 uL
Reagent
7.5 uL
Master Mix (with 10% overage)
Total DNA16.50 ug (16.5 uL)
Total Reagent49.5 uL
Reduced Serum Media660 uL
Protocol
- 1. DNA Tube: Dilute 16.5 uL DNA in 330 uL OptiMEM
- 2. Reagent Tube: Dilute 49.5 uL reagent in 330 uL OptiMEM
- 3. Combine: Add diluted DNA to diluted reagent, mix gently
- 4. Incubate: 15-20 min at room temperature
- 5. Add to cells: 200 uL complex per well
Well Information
Growth Area9.6 cm2
DNA Density0.26 ug/cm2
Reagent Ratio3:1 (uL:ug)
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Editorial Note
MyCalcBuddy Editorial Team
This page is maintained as an educational calculator reference.
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Formula Source: Standard Mathematical References
by Various
🔄Last reviewed: May 2026
✓Formula checks are based on standard references and internal QA review.