Transfection Calculator

Calculate DNA and reagent amounts for cell transfection experiments.

Transfection Parameters

Plate Format

Number of Wells/Dishes

DNA per Well (ug)

Reagent:DNA Ratio (uL:ug)

DNA Stock Concentration (ng/uL)

Media Volume per Well (uL)

Per Well Requirements

DNA

2.5 ug

2.5 uL

Reagent

7.5 uL

Master Mix (with 10% overage)

Total DNA16.50 ug (16.5 uL)

Total Reagent49.5 uL

Reduced Serum Media660 uL

Protocol

1. DNA Tube: Dilute 16.5 uL DNA in 330 uL OptiMEM
2. Reagent Tube: Dilute 49.5 uL reagent in 330 uL OptiMEM
3. Combine: Add diluted DNA to diluted reagent, mix gently
4. Incubate: 15-20 min at room temperature
5. Add to cells: 200 uL complex per well

Well Information

Growth Area9.6 cm2

DNA Density0.26 ug/cm2

Reagent Ratio3:1 (uL:ug)