BCA Assay Calculator
Calculate protein concentration from BCA assay with statistical analysis
Standard Curve (BSA)
Concentration (µg/mL)Absorbance (562nm)
Sample Replicates
Sample Concentration
624.2 µg/mL
± 3.3 µg/mL
Statistical Analysis
Mean Absorbance0.8500
Std Deviation0.0041
CV%0.48%
Curve Fit
Equationy = 0.001220x + 0.0887
R²0.9964
Working Range
BCA assay linear range: 25-2000 µg/mL (standard protocol) or 0.5-20 µg/mL (enhanced protocol)
About BCA Assay
The BCA (Bicinchoninic Acid) assay is a colorimetric protein quantification method based on Cu²⁺ reduction to Cu⁺ in alkaline conditions, followed by BCA chelation producing a purple color.
- Detection range: 20-2000 µg/mL (standard) or 0.5-20 µg/mL (micro)
- Wavelength: 562 nm
- Advantages: Compatible with detergents, stable color, wide dynamic range
- Limitations: Temperature-sensitive, reducing agent interference