Bradford Assay Calculator
Calculate protein concentration from Bradford assay with automatic standard curve fitting
Standard Curve Data
Concentration (µg/mL)Absorbance (595nm)
Sample Measurement
Sample Concentration
432.2 µg/mL
Standard Curve
Equationy = 0.000939x + 0.0442
R²0.9997
Slope0.000939
Y-Intercept0.0442
Visual Standard Curve
0Concentration (µg/mL)1000
About Bradford Assay
The Bradford assay is a colorimetric protein quantification method based on the binding of Coomassie Brilliant Blue G-250 dye to proteins.
- Detection range: 1-1500 µg/mL (standard) or 1-25 µg/mL (micro)
- Wavelength: 595 nm
- Advantages: Fast, sensitive, minimal interference from common reagents
- Limitations: Protein-to-protein variation, detergent interference