Lowry Assay Calculator
Calculate protein concentration from Lowry assay with automatic standard curve fitting
Standard Curve Data (BSA)
Concentration (µg/mL)Absorbance (750nm)
Sample Measurement
Sample Concentration
166.0 µg/mL
Standard Curve Parameters
Equationy = 0.002215x + 0.0124
R²0.9998
CorrelationExcellent
Important Notes
- • Read absorbance within 30 minutes of color development
- • Linear range typically 5-100 µg/mL
- • Reducing agents interfere with the assay
About Lowry Assay
The Lowry protein assay is a biochemical assay for determining the total level of protein in a solution. It combines the Biuret reaction (Cu²⁺ binding to peptide bonds) with Folin-Ciocalteu reagent reduction by aromatic residues.
- Detection range: 5-100 µg/mL (standard), 1-25 µg/mL (micro)
- Wavelength: 750 nm (or 660 nm)
- Advantages: High sensitivity, well-established method
- Limitations: Time-consuming, subject to interference