RNA Molecular Weight Calculator
Calculate the molecular weight of RNA sequences for laboratory applications
Input Parameters
Use U for uracil (RNA), not T
Nucleotide Molecular Weights
AMP (Adenosine)347.22 Da
UMP (Uridine)324.18 Da
GMP (Guanosine)363.22 Da
CMP (Cytidine)323.20 Da
Molecular Weight
4220.50 Da
4.221 kDa
Detailed Results
Length13 nt
MW (unmodified)4220.50 Da
GC Content53.8%
Extinction Coef. (260nm)144,100 M⁻¹cm⁻¹
Mass for 1 nmol4.221 µg
Mass for 1 pmol4.221 ng
Base Composition
3
Adenine
3
Uracil
4
Guanine
3
Cytosine
About RNA Molecular Weight
RNA molecular weight differs from DNA due to the presence of a 2'-hydroxyl group on the ribose sugar and uracil instead of thymine.
Key considerations:
- RNA nucleotides are approximately 16 Da heavier than corresponding DNA nucleotides
- Average MW per nucleotide: ~340 Da (vs ~330 Da for DNA)
- 5' modifications like caps significantly increase the total molecular weight
- Poly-A tails on mRNA add ~347 Da per adenosine