PCR Annealing Temperature Calculator
Determine optimal annealing temperature from primer Tm values
Primer Tm Values
Annealing Temperature (Ta)
54.0°C
Average Tm - 5°C
Tm Analysis
Average Tm59.0°C
Lower Tm58.0°C
Higher Tm60.0°C
Tm Difference2.0°C
All Ta Methods
Average - 5°C54.0°C
Lower - 5°C53.0°C
Optimal55.0°C
Touchdown Start63.0°C
Gradient PCR Range
48.0°C → 65.0°C
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Editorial Note
MyCalcBuddy Editorial Team
This page is maintained as an educational calculator reference.
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Formula Source: Standard Mathematical References
by Various
🔄Last reviewed: May 2026
✓Formula checks are based on standard references and internal QA review.