PCR Cycle Number Calculator
Determine optimal number of PCR cycles for your experiment
PCR Parameters
Number of target DNA copies
50%95%100%
PCR Efficiency Guide
- • Excellent: 95-100%
- • Good: 90-95%
- • Acceptable: 80-90%
- • Poor: <80%
Recommended Cycles
21
Range: 20 - 26 cycles
Calculation Results
Theoretical (100% eff.)19.9 cycles
At 95% efficiency20.7 cycles
Expected Yield1.23 billion
Amplification Factor1.23 millionx
Yield by Cycle Number
25 cycles17.82 billion
30 cycles502.39 billion
35 cycles14.16 trillion
40 cycles399.38 trillion