PCR Extension Time Calculator
Determine optimal extension time based on amplicon length and polymerase
PCR Parameters
Extension Guidelines
- • Short amplicons (<500 bp): 15-30 seconds
- • Medium amplicons (500-2000 bp): 30-60 seconds
- • Long amplicons (>2000 bp): 1-2 min per kb
- • Add 10-30% extra time for reliability
Recommended Extension Time
1 sec
at 72°C
Extension Details
PolymeraseTaq Polymerase
Extension Rate1000 bp/s
Calculated Time1 sec
Minimum Time30 sec
Optimal Temperature72°C