Spectrophotometer Calculator
Calculate concentration from UV-Vis spectrophotometer measurements
Measurement Parameters
Concentration
10.936 µM
1.094e-5 M
Measurement Results
Corrected Absorbance0.4800
Transmittance33.11%
Light Absorbed66.89%
Signal/Noise Ratio24.0
Quality Check
Absorbance is within the linear range (0.1-1.0)
Common Wavelengths
About Spectrophotometry
Spectrophotometry measures light absorption by samples at specific wavelengths. Key considerations:
- Linear range: A = 0.1-1.0 for accurate measurements
- Blank correction: Always subtract buffer/solvent background
- Path length: Standard cuvettes are 1 cm; nanodrop uses ~1 mm
- Wavelength selection: Choose wavelength of maximum absorption (λmax)